To avoid the risk of the recognition region being hidden within the protein, it is usually recommended to select the N and C ends of the protein to produce the corresponding antibody. Because in a complete protein, the N and C ends are usually exposed to the surface of the protein. However, it must be noted that the C-terminus of the membrane protein is too hydrophobic to be suitable as an antigen.

The molecular weight (MW) of the peptide antigen (unbound) is too small to be used in traditional western blotting (WB) tests. To verify the specificity of target bands in WB, it is recommended to run an "antibody block" control and use peptide antigen to block the primary antibody. The recommended bleeding dilution is 1:1000 (equal to 1µg/ml) and the blocking peptide is about 2µg/ml.

A quality antibody is one that has the appropriate sensitivity and specificity to answer the questions your test poses. In addition, one antibody is not necessarily suitable for all applications, as each application has different performance specifications.

Both polyclonal and monoclonal antibodies have their own advantages. Generally speaking, monoclonal antibodies have strong specificity, but relatively small affinity, and relatively low sensitivity to detect antigens. The specificity of polyclonal antibody is weak, but the affinity of the antibody is strong and the sensitivity is high. Compared to monoclonal antibodies, large quantities of polyclonal antibodies are relatively quick and inexpensive to produce, and they are nonspecific because they are able to recognize multiple epitopes on any one antigen.

For qualified protein antigens, Qiangyao can guarantee two ELISA and WB positive clones against the immunogen. For qualified peptide antigens, Qiangyao can guarantee two positive clones, the ELISA results against the immunogen are positive. For other cases, Qiangyao will provide assurance based on the immune response after immunization.

Qiangyao Biotechnology Co., Ltd. has successfully built a complete platform of various protein expression technologies, including prokaryotic protein expression and purification, yeast protein expression and purification, insect cell protein expression and purification, mammalian cell protein expression and purification.

With more than 10 years of experience in protein expression and a successful delivery rate of over 96%, we provide one-stop services from gene synthesis, vector construction, gene expression, protein purification and detection to meet customers' individual needs.

Different protein expression systems are selected according to the customer protein source (bacterial, animal or other) and the purpose of the purified protein. If you do not know how to choose, please consult online customer service, we will wholeheartedly recommend the right expression system for customers.

<50aa is generally used for peptide synthesis, >50aa peptide synthesis is difficult to try to express.

Impurities are mostly in cells or in the medium and can be removed by chromatography.